GRADUATE SCHOOL
M.SC. in Bioengineering (With Thesis)
BEN 509 | Course Introduction and Application Information
| Course Name |
Enzyme Kinetics
|
|
Code
|
Semester
|
Theory
(hour/week) |
Application/Lab
(hour/week) |
Local Credits
|
ECTS
|
|
BEN 509
|
Fall/Spring
|
2
|
1
|
3
|
7.5
|
| Prerequisites |
None
|
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| Course Language |
English
|
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| Course Type |
Elective
|
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| Course Level |
Second Cycle
|
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| Mode of Delivery | - | |||||
| Teaching Methods and Techniques of the Course | - | |||||
| Course Coordinator | ||||||
| Course Lecturer(s) | ||||||
| Assistant(s) | - | |||||
| Course Objectives | This course aims to inform the students about enzyme sources, types, structure and functions, properties, factors affecting the activity of enzymes, beneficial effects of enzymes, teaching ways of protecting from negative effects, new developments in enzyme science and technology. |
| Learning Outcomes |
The students who succeeded in this course;
|
| Course Description | This course covers chemical structure and properties of enzymes. Enzyme specificity. Enzyme types and functions. Enzymatic reactions and enzyme kinetics. Factors affecting enzyme activity. Enzyme stability and prophylaxis. The use of new developments and engineering knowledge in enzyme science and technology in enzyme technology. Immobilized enzymes, immobilization techniques and application areas. |
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|
Core Courses | |
| Major Area Courses | ||
| Supportive Courses |
X
|
|
| Media and Management Skills Courses | ||
| Transferable Skill Courses |
WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES
| Week | Subjects | Related Preparation |
| 1 | Introduction; Enzymes | Jey, J.E., Ollis, D. F., Biochemical Engineering Fundamentals, Mc-Graw Hill, 1986. |
| 2 | Three-dimensional structure of enzymes | Bugg, T. D. H. Introduction to Enzyme and Coenzyme Chemistry 3rd Edition, United Kingdom: John Wiley & Sons; 2012. |
| 3 | Structure of enzyme- substrate complexes | Marangoni, A.G., Enzyme Kinetics: A Modern Approach, John Wiley and Sons, 2003. |
| 4 | Kinetic analysis techniques | Copeland, C.A., Enzymes: A practical introduction to structure, mechanism and data analysis, Wiley-VCH Inc., 2000. |
| 5 | Transition state theory Principles of catalysis | Copeland, C.A., Enzymes: A practical introduction to structure, mechanism and data analysis, Wiley-VCH Inc., 2000. |
| 6 | How do enzymes work? | Marangoni, A.G., Enzyme Kinetics: A Modern Approach, John Wiley and Sons, 2003.. |
| 7 | Characterization of enzyme activity | Copeland, C.A., Enzymes: A practical introduction to structure, mechanism and data analysis, Wiley-VCH Inc., 2000. |
| 8 | Enzyme kinetics | Marangoni, A.G., Enzyme Kinetics: A Modern Approach, John Wiley and Sons, 2003. |
| 9 | Stereochemistry of enzymatic reactions | Chapter-10, ‘‘Food Process Design’’, Zacharias B. Maroulis, George D. Saravacos, Marcel Dekker, Inc., 2003. |
| 10 | Cooperative ligand binding, allosteric interactions, regulation | Copeland, C.A., Enzymes: A practical introduction to structure, mechanism and data analysis, Wiley-VCH Inc., 2000. |
| 11 | Reversible enzyme inhibition kinetics | Marangoni, A.G., Enzyme Kinetics: A Modern Approach, John Wiley and Sons, 2003. |
| 12 | Irreversible enzyme inhibition kinetics | Marangoni, A.G., Enzyme Kinetics: A Modern Approach, John Wiley and Sons, 2003. |
| 13 | Enzyme kinetics pH dependence of catalysis | Marangoni, A.G., Enzyme Kinetics: A Modern Approach, John Wiley and Sons, 2003. |
| 14 | Two substrate reactions | Bailey, J.E., Ollis, D. F., Biochemical Engineering Fundamentals, Mc-Graw Hill, 1986. |
| 15 | Enzyme immobilization, advantages and disadvantages | Bailey, J.E., Ollis, D. F., Biochemical Engineering Fundamentals, Mc-Graw Hill, 1986. |
| 16 | Enzyme immobilization methods | Bailey, J.E., Ollis, D. F., Biochemical Engineering Fundamentals, Mc-Graw Hill, 1986. |
| Course Notes/Textbooks | Depending on the topic selected, resources will be provided by the responsible faculty member. |
| Suggested Readings/Materials |
|
EVALUATION SYSTEM
| Semester Activities | Number | Weigthing |
| Participation |
1
|
10
|
| Laboratory / Application |
1
|
25
|
| Field Work | ||
| Quizzes / Studio Critiques | ||
| Portfolio | ||
| Homework / Assignments |
1
|
20
|
| Presentation / Jury |
1
|
10
|
| Project |
1
|
15
|
| Seminar / Workshop | ||
| Oral Exams | ||
| Midterm | ||
| Final Exam |
1
|
20
|
| Total |
| Weighting of Semester Activities on the Final Grade |
5
|
80
|
| Weighting of End-of-Semester Activities on the Final Grade |
1
|
20
|
| Total |
ECTS / WORKLOAD TABLE
| Semester Activities | Number | Duration (Hours) | Workload |
|---|---|---|---|
| Theoretical Course Hours (Including exam week: 16 x total hours) |
16
|
2
|
32
|
| Laboratory / Application Hours (Including exam week: '.16.' x total hours) |
16
|
1
|
16
|
| Study Hours Out of Class |
16
|
5
|
80
|
| Field Work |
0
|
||
| Quizzes / Studio Critiques |
0
|
||
| Portfolio |
0
|
||
| Homework / Assignments |
1
|
20
|
20
|
| Presentation / Jury |
1
|
20
|
20
|
| Project |
1
|
27
|
27
|
| Seminar / Workshop |
0
|
||
| Oral Exam |
0
|
||
| Midterms |
0
|
||
| Final Exam |
1
|
30
|
30
|
| Total |
225
|
COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP
|
#
|
Program Competencies/Outcomes |
* Contribution Level
|
||||
|
1
|
2
|
3
|
4
|
5
|
||
| 1 | To be able to have adequate knowledge in Mathematics, Life Sciences and Bioengineering; to be able to use theoretical and applied information in these areas to model and solve Bioengineering problems. |
X | ||||
| 2 | To be able to use scientific methods to complete and apply information from uncertain, limited or incomplete data; to be able to combine and use information from related disciplines. |
X | ||||
| 3 | To be able to design and apply theoretical, experimental and model-based research; to be able to solve complex problems in such processes. |
X | ||||
| 4 | Being able to utilize Natural Sciences and Bioengineering principles to design systems, devices and processes. |
X | ||||
| 5 | To be able to follow and apply new developments and technologies in the field of Bioengineering. |
|||||
| 6 | To be able to work effectively in multi-disciplinary teams within the discipline of Bioengineering; to be able to exhibit individual work. |
X | ||||
| 7 | To be able to have the knowledge about the social, environmental, health, security and law implications of Bioengineering applications, to be able to have the knowledge to manage projects and business applications, and to be able to be aware of their limitations in professional life. |
X | ||||
| 8 | To be able to have the social, scientific and ethical values in the stages of collection, interpretation, dissemination and application of data related to the field of Bioengineering. |
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| 9 | To be able to prepare an original thesis/term project in accordance with the criteria related to the field of Bioengineering. |
X | ||||
| 10 | To be able to follow information about Bioengineering in a foreign language and to be able to participate in discussions in academic environments. |
|||||
| 11 | To be able to improve the acquired knowledge, skills and qualifications for social and universal purposes regarding the studied area. |
X | ||||
| 12 | To be able to recognize regional and global issues/problems, and to be able to develop solutions based on research and scientific evidence related to Bioengineering. |
X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest