GRADUATE SCHOOL
M.SC. in Bioengineering (With Thesis)
BEN 515 | Course Introduction and Application Information
| Course Name |
Instrumental Analysis of Biomolecules
|
|
Code
|
Semester
|
Theory
(hour/week) |
Application/Lab
(hour/week) |
Local Credits
|
ECTS
|
|
BEN 515
|
Fall/Spring
|
3
|
0
|
3
|
7.5
|
| Prerequisites |
None
|
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| Course Language |
English
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| Course Type |
Elective
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| Course Level |
Second Cycle
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| Mode of Delivery | - | |||||
| Teaching Methods and Techniques of the Course | - | |||||
| Course Coordinator | ||||||
| Course Lecturer(s) | ||||||
| Assistant(s) | - | |||||
| Course Objectives | The main aim is to explain the analytical methods and instruments to investigate structures of biomolecules, their activities, their interaction with other biomolecules or inhibitors. We will discuss the production of the biomolecule of interest, methods to purify these molecules of interest, methods to investigate their structures and characterize them. |
| Learning Outcomes |
The students who succeeded in this course;
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| Course Description |
|
|
Core Courses | |
| Major Area Courses |
X
|
|
| Supportive Courses | ||
| Media and Management Skills Courses | ||
| Transferable Skill Courses |
WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES
| Week | Subjects | Related Preparation |
| 1 | Introduction to Biomolecules | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 1 |
| 2 | Special Chemical Requirements of Biomolecules, Factors Affecting Structure and Stability | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 1 |
| 3 | Principles of Chromatography | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 2 |
| 4 | Modes of Chromatography: Ion Exchange, Gel Filtration, Reversed Phase | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 2 |
| 5 | Modes of Chromatography: Hydrophobis Interaction and Affinity Chromatography | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 2 |
| 6 | Chromatographic Separation: HPLC | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 2 |
| 7 | Electrophoresis | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 5 |
| 8 | Midterm | |
| 9 | Spectroscopic Techniques:Ultraviolet/Visible Absorption Spectroscopy | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 3 |
| 10 | Spectroscopic Techniques: Fluorescence Spectroscopy | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 3 |
| 11 | Spectroscopic Techniques: Techniques Using Plane-Polarized Light | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 3 |
| 12 | Spectroscopic Techniques: Nuclear Magnetic Resonance (NMR) Spectroscopy | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 3 |
| 13 | Mass Spectrometry | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 4 |
| 14 | Hydrodynamic Methods | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 7 |
| 15 | Current Applications and Presentations | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). Chapter 8 |
| 16 | Final |
| Course Notes/Textbooks | Sheehan, D. ,2009. Physical Biochemistry: Principles And Applications. ISBNs: 9780470856024 (HB). |
| Suggested Readings/Materials | Structure and Mechanism in Protein Science by Fersht Introduction to Protein Structure, by Branden and Tooze Biochemistry by Voet and Voet |
EVALUATION SYSTEM
| Semester Activities | Number | Weigthing |
| Participation | ||
| Laboratory / Application | ||
| Field Work | ||
| Quizzes / Studio Critiques | ||
| Portfolio | ||
| Homework / Assignments | ||
| Presentation / Jury |
1
|
25
|
| Project | ||
| Seminar / Workshop | ||
| Oral Exams | ||
| Midterm |
1
|
35
|
| Final Exam |
1
|
40
|
| Total |
| Weighting of Semester Activities on the Final Grade |
2
|
60
|
| Weighting of End-of-Semester Activities on the Final Grade |
1
|
40
|
| Total |
ECTS / WORKLOAD TABLE
| Semester Activities | Number | Duration (Hours) | Workload |
|---|---|---|---|
| Theoretical Course Hours (Including exam week: 16 x total hours) |
16
|
3
|
48
|
| Laboratory / Application Hours (Including exam week: '.16.' x total hours) |
16
|
0
|
|
| Study Hours Out of Class |
16
|
5
|
80
|
| Field Work |
0
|
||
| Quizzes / Studio Critiques |
0
|
||
| Portfolio |
0
|
||
| Homework / Assignments |
0
|
||
| Presentation / Jury |
1
|
15
|
15
|
| Project |
0
|
||
| Seminar / Workshop |
0
|
||
| Oral Exam |
0
|
||
| Midterms |
1
|
17
|
17
|
| Final Exam |
1
|
20
|
20
|
| Total |
180
|
COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP
|
#
|
Program Competencies/Outcomes |
* Contribution Level
|
||||
|
1
|
2
|
3
|
4
|
5
|
||
| 1 | To be able to have adequate knowledge in Mathematics, Life Sciences and Bioengineering; to be able to use theoretical and applied information in these areas to model and solve Bioengineering problems. |
X | ||||
| 2 | To be able to use scientific methods to complete and apply information from uncertain, limited or incomplete data; to be able to combine and use information from related disciplines. |
X | ||||
| 3 | To be able to design and apply theoretical, experimental and model-based research; to be able to solve complex problems in such processes. |
X | ||||
| 4 | Being able to utilize Natural Sciences and Bioengineering principles to design systems, devices and processes. |
X | ||||
| 5 | To be able to follow and apply new developments and technologies in the field of Bioengineering. |
X | ||||
| 6 | To be able to work effectively in multi-disciplinary teams within the discipline of Bioengineering; to be able to exhibit individual work. |
X | ||||
| 7 | To be able to have the knowledge about the social, environmental, health, security and law implications of Bioengineering applications, to be able to have the knowledge to manage projects and business applications, and to be able to be aware of their limitations in professional life. |
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| 8 | To be able to have the social, scientific and ethical values in the stages of collection, interpretation, dissemination and application of data related to the field of Bioengineering. |
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| 9 | To be able to prepare an original thesis/term project in accordance with the criteria related to the field of Bioengineering. |
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| 10 | To be able to follow information about Bioengineering in a foreign language and to be able to participate in discussions in academic environments. |
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| 11 | To be able to improve the acquired knowledge, skills and qualifications for social and universal purposes regarding the studied area. |
X | ||||
| 12 | To be able to recognize regional and global issues/problems, and to be able to develop solutions based on research and scientific evidence related to Bioengineering. |
X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest